CD36 Monoclonal Antibody (HM36), Alexa Fluor™ 700, eBioscience™, Invitrogen™

Description

• Description: This HM36 antibody recognizes mouse CD36, also known as scavenger receptor BIII
• Applications Reported: This HM36 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This HM36 antibody has been tested by flow cytometric analysis of thioglycolate-elicited peritoneal macrophages
• This may be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633-647 nm)
• Most instruments will require a 685 LP mirror and 710/20 band pass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser CD36 (fatty acid translocase, FAT) is an 88 kDa, ditopic glycosylated protein that belongs to the class B family of scavenger receptors
• CD36 is expressed by most resting marginal zone B cells but not by follicular and B1 B cells, and is rapidly induced on Follicular B cells in vitro upon TLR and CD40 stimulation
• CD36 does not affect the development of B cells, but modulates both primary and secondary antibody response
• Similar to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin
• In mouse, CD36 is responsible for gustatory perception of long-chain fatty acids
• CD36 is preferentially found within lipid rafts, which facilitates its association with receptors, signaling and adapter molecules
• CD36 binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL
• Further, CD36 may function as a cell adhesion molecule and directly mediates cyto-adherence of Plasmodium falciparum parasitized erythrocytes
• Mutations in the CD36 gene cause platelet glycoprotein deficiency
• Multiple alternatively spliced transcript variants encoding the CD36 protein have been found.