CD44 Monoclonal Antibody (IM7), Alexa Fluor™ 532, eBioscience™, Invitrogen™

Description

• Description: The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1)
• CD44 is expressed by hematopoietic and non-hematopoietic cells
• Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44
• CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component
• Applications Reported: This IM7 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This IM7 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This may be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Alexa Fluor 532 is excited with the Green laser (532 nm) and emits at 561 nm
• This cannot be used with the Yellow-Green laser (561 nm)
• We recommend using a 560/14 band pass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast
• Several isoforms of CD44 exist, including the predomit CD44H isoform detected in many normal tissues
• CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration
• CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing
• CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential
• CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes
• Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events
• Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined
• Alternative splicing is the basis for the structural and functional diversity of the CD44 protein
• Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus
• CD44 also may be related to tumor metastasis formation.