CD71 (Transferrin Receptor) Monoclonal Antibody (R17217 (RI7 217.1.4)), FITC, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD71 (Transferrin Receptor)
Concentration
0.5 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
2610028K12Rik; AI195355; AI426448; AU015758; CD antigen CD71; CD71; chicken transferrin receptor; E430033M20Rik; I79_000642; IMD46; mammary tumor virus receptor 1; Mtvr1; Mtvr-1; p90; sTfR; T9; TfR; tfR1; TFR2; TFRC; TR; transferrin receptor; transferrin receptor (p90, CD71); transferrin receptor 2; transferrin receptor protein 1; Transferrin receptor protein 1, serum form; Trfr
Gene Symbols
TFRC
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
TFRC
Clone
R17217 (RI7 217.1.4)
Applications
Flow Cytometry
Conjugate
FITC
Host Species
Rat
Target Species
Mouse
Gene Accession No.
Q62351
Gene ID (Entrez)
22042
Isotype
IgG2a κ
Purification Method
Affinity chromatography
Product Type
Antibody
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Description
- Description: The R17217 monoclonal antibody reacts with mouse CD71, a 170-180 kDa type II transmembrane protein
- CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth
- CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes
- Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71
- Expression level differences have been observed in different mouse strains
- Applications Reported: The R17217 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This R17217 antibody has been tested by flow cytometric analysis of mouse bone marrow cells
- This can be used at less than or equal to 0.5 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
- Filtration: 0.2 μm post-manufacturing filtered
- Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes
- Endosomal acidification leads to iron release
- The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor
- Transferrin receptor is necessary for development of erythrocytes and the nervous system
- Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.