CD54 (ICAM-1) Monoclonal Antibody (HA58), PE, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD54 (ICAM-1)
Concentration
5 μL/Test
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias
BB2; CD54; cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM; Icam1; ICAM-1; ICAM-1; CD54 homolog; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule-1; intercellular adhesion molecule-1 precursor; Ly 47; Ly-47; major group rhinovirus receptor; MALA2; MALA-2; MyD10; P3.58; sCD54; soluble CD54; Surface antigen of activated B cells
Gene Symbols
ICAM1
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
ICAM1
Clone
HA58
Applications
Flow Cytometry
Conjugate
PE
Host Species
Mouse
Target Species
Human
Gene Accession No.
P05362
Gene ID (Entrez)
3383
Isotype
IgG1 κ
Purification Method
Affinity chromatography
Product Type
Antibody
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Description
- Description: The HA58 monoclonal antibody reacts with human CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes and endothelial cells
- Expression of CD54 is upregulated on activated lymphocytes
- Interaction of CD54 with its ligand CD11a is important in the inflammatory response
- Applications Reported: The HA58 antibody has been reported for use in flow cytometric analysis
- Applications Tested: The HA58 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
- This can be used at 5 μL (0.25 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
- Filtration: 0.2 μm post-manufacturing filtered
- ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain
- ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102)
- ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor
- ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation
- ICAM-1 is also detected in some carcinoma and melanoma cells
- Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.