CD105 (Endoglin) Monoclonal Antibody (SN6), PE, eBioscience™, Invitrogen™

Description

• Description: The SN6 monoclonal antibody reacts with human CD105, also known as Endoglin
• CD105, an approximately 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells and some bone marrow cells and activated macrophages
• It is suggested that CD105 functions in adhesion and embryonic angiogenesis
• Applications Reported: The SN6 antibody has been reported for use in flow cytometric analysis
• Applications Tested: The SN6 antibody has been pre-titrated and tested by flow cytometric analysis of the U937 cell line
• This can be used at 5 μL (1 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells
• It exists on an O- and N-glycosylated homodimer
• Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker
• CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.