7069354
CD105 (Endoglin) Monoclonal Antibody (MJ7/18), eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD105 (Endoglin)
Concentration
0.5 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
AI528660; AI662476; CD105; CD105 antigen; cell surface MJ7/18 antigen; Edg; END; Endo; Endoglin; endoglin (Osler-Rendu-Weber syndrome 1); Eng; HHT1; ORW; ORW1; S-endoglin; transmembrane glycoprotein
Gene Symbols
ENG
Primary or Secondary
Primary
Content And Storage
4° C
Gene
ENG
Clone
MJ7/18
Applications
Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Host Species
Rat
Target Species
Mouse
Gene Accession No.
Q63961
Gene ID (Entrez)
13805
Isotype
IgG2a κ
Purification Method
Affinity chromatography
Product Type
Antibody
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Description
- Description: The MJ7/18 monoclonal antibody reacts with the mouse CD105 molecule, also known as Endoglin
- This 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells
- It is suggested that CD105 functions in adhesion and embryonic angiogenesis
- Applications Reported: The MJ7/18 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections
- Applications Tested: The MJ7/18 antibody has been tested by flow cytometric analysis of bEnd.3 cells or mouse splenocytes
- This can be used at less than or equal to 0.5 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Purity: Greater than 90%, as determined by SDS-PAGE
- Aggregation: Less than 10%, as determined by HPLC
- Filtration: 0.2 μm post-manufacturing filtered
- CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells
- It exists on an O- and N-glycosylated homodimer
- Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker
- CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.