7069355
CD106 (VCAM-1) Monoclonal Antibody (eBioMR106 (MR106)), eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD106 (VCAM-1)
Concentration
0.5 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
adhesion molecule VCAM-1; CD106; CD106 antigen; DKFZp779G2333; INCAM100; INCAM-100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; vascular cell adhesion molecule; vascular cell adhesion molecule 1; vascular cell adhesion molecule-1; vascular cell adhesion protein 1; V-CAM 1; Vcam1; Vcam-1; VCAM1B
Gene Symbols
VCAM1
Primary or Secondary
Primary
Content And Storage
4° C
Gene
VCAM1
Clone
eBioMR106 (MR106)
Applications
Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Target Species
Rat
Gene Accession No.
P29534
Gene ID (Entrez)
25361
Isotype
IgG1 κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The eBioMR106 monoclonal antibody reacts with rat CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells
- Endothelial cells constitutively express low levels of CD106 and further upregulate VCAM-1 upon cytokine stimulation
- CD106 binds to integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin alpha4beta7 (LPAM-1) and these interactions in the bone marrow and thymus are important for early lymphocyte and myeloid development
- Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response
- Applications Reported: This eBioMR106 (MR106) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistology staining of frozen tissue sections
- Applications Tested: This eBioMR106 (MR106) antibody has been tested by flow cytometric analysis of rat bone marrow cells
- This can be used at less than or equal to 0.5 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Purity: Greater than 90%, as determined by SDS-PAGE
- VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium
- The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.