Plexin-B2 Monoclonal Antibody (eBio3E7 (3E7)), eBioscience™, Invitrogen™

Description

• Description: Plexin-B2 is a member of the Semaphorin receptor family
• The semaphorin family consists of membrane and secreted proteins involved in attraction and repulsion of neuronal guidance cues
• Plexin-B1 is a transmembrane protein that can be cleaved coverting the single chain presursor into a heterodimeric receptor
• It has been shown to bind Sema4D/CD100 and is involved in axon development in mouse embryos
• Sema4D also plays a role in the immune system interacting with CD72
• Plexin-B2's possible role in the immune system has not been characterized
• Applications Reported: This eBio3E7 (3E7) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining
• Applications Tested: This eBio3E7 (3E7) antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• Plexin-B2 is a member of the Semaphorin receptor family
• The semaphorin family consists of membrane and secreted proteins involved in attraction and repulsion of neuronal guidance cues
• Plexin-B1 is a transmembrane protein that can be cleaved converting the single chain precursor into a heterodimeric receptor
• It has been shown to bind Sema4D/CD100 and is involved in axon development in mouse embryos
• Sema4D also plays a role in the immune system interacting with CD72
• Plexin-B2's possible role in the immune system has not been characterized.