Ea52-68 peptide bound to I-Ab Monoclonal Antibody (eBioY-Ae (YAe, Y-Ae)), eBioscience™, Invitrogen™

Description

• Description: The Y-Ae antibody reacts with a class II major histocompatibility complex (MHC) self Ea peptide (peptide 52-68) bound to I-Ab molecules
• The Y-Ae antibody detects a determit expressed on a subset of class II I-Ab molecules in strains that also express class II I-Eb
• This determit is expressed on peripheral B cells and on cells in the thymic medulla, but not thymic cortical epithelium
• In addition to B cells, the Y-Ae determit is expressed at comparable levels on other antigen presenting cells, including macrophages and dendritic cells
• The antibody does not react with invariant chain-associated class II MHC complexes
• The reactivity of the Y-Ae antibody is MHC-restricted
• The Y-Ae antibody was generated by immunization of [B10.MBR x B10.D2]F1 recipients with B10A(5R) LPS blasts
• In this strain combination, there is incompatibility for I-Ab and I-Eb molecules
• The antibody reacts with 10-15% of surface I-Ab molecules in strains that also express surface I-Eb molecules (e.g., strains B10.A(5R) and B10.A(3R))
• The antibody does not react with I-A molecules in strains that have a non-functional I-E (a chain) gene (e.g., strains B6 and B10), nor with mutant I-A molecules (e.g., (B6.c-H-2bm-12 x A.TFR5)F1), nor with any other strain tested
• The Y-Ae antibody recognizes an Ea peptide (Ea 52-68) bound to I-Ab molecules, but not to Ea 52-68 peptide bound to I-Ak or I-Abm-12 molecules
• MHC class II molecules are heterodimers of non-covalently associated alpha (31-34 kDa) and beta (26-29 kDa) chains
• Major histocompatibility complex class II antigen presentation requires the participation of lysosomal proteases in two convergent processes
• First, the antigens endocytosed by the antigen presenting cells must be broken down into antigenic peptides
• Second, class II molecules are synthesized with their peptide-binding site blocked by invariant chain (Ii), and they aquire the capacity to bind antigens only after Ii has been degraded in the compartments where peptides reside
• MHC class II molecules present exogenously derived antigen to CD4+ T lymphocytes, which are usually T helper cells
• CD4 interacts with non-polymorphic residues of MHC class II
• HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.