7069462
Aiolos Monoclonal Antibody (8B2), eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Aiolos
Concentration
0.5 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
5830411O07Rik; AIO; AIOLOS; IKAROS family zinc finger 3; IKAROS family zinc finger 3 (Aiolos); ikaros family zinc finger protein 3; Ikzf3; Zfpn1a3; zinc finger DNA binding protein Aiolos; zinc finger protein Aiolos; zinc finger protein, subfamily 1A, 3 (Aiolos); Znfn1a3
Gene Symbols
Ikzf3
Primary or Secondary
Primary
Content And Storage
4° C
Gene
Ikzf3
Clone
8B2
Applications
Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Target Species
Mouse
Gene Accession No.
O08900
Gene ID (Entrez)
22780
Isotype
IgG1 κ
Purification Method
Affinity chromatography
Product Type
Antibody
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Description
- Description: This 8B2 monoclonal antibody reacts with mouse Aiolos, a 58-kDa member of the Ikaros family of zinc finger transcription factors
- Expression of Aiolos is restricted to the lymphoid lineage, with B cells exhibiting the highest levels of expression, followed by NK and T cells
- Studies demonstrate that Aiolos forms homodimers or heterodimerizes with Ikaros proteins
- Moreover, Aiolos exhibits tyrosine phosphorylation and nuclear localization in response to IL-2 stimulation in T cells
- Aiolos plays a role in IL-2-mediated anti-apoptosis by controlling Bcl-2 expression
- Finally, Aiolos is involved in B cell development, with upregulation of this transcription factor observed at the pre-BI to pre-BII transition in response to pre-B cell receptor signaling
- Applications Reported: This 8B2 antibody has been reported for use in immunoblotting/western blotting
- Applications Tested: This 8B2 antibody has been tested by immunoblotting of reduced cell lysate prepared from mouse splenocytes
- This can be used at less than or equal to 5 μg/mL
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Purity: Greater than 90%, as determined by SDS-PAGE
- Aggregation: Less than 10%, as determined by HPLC
- Filtration: 0.2 μm post-manufacturing filtered
- This gene encodes a member of the Ikaros family of zinc-finger proteins
- Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development
- This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation
- Both Ikaros and Aiolos can participate in chromatin remodeling
- Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers
- At least six alternative transcripts encoding different isoforms have been described.