7069855
CD106 (VCAM-1) Monoclonal Antibody (429), Functional Grade, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
CD106 (VCAM-1)
Concentration
1 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with no preservative; pH 7.2
Gene Alias
adhesion molecule VCAM-1; CD106; CD106 antigen; DKFZp779G2333; INCAM100; INCAM-100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; vascular cell adhesion molecule; vascular cell adhesion molecule 1; vascular cell adhesion molecule-1; vascular cell adhesion protein 1; V-CAM 1; Vcam1; Vcam-1; VCAM1B
Gene Symbols
VCAM1
Primary or Secondary
Primary
Content And Storage
4° C
Gene
VCAM1
Clone
429
Applications
Flow Cytometry, Functional Assay, Neutralization
Conjugate
Functional Grade
Host Species
Rat
Target Species
Mouse
Gene Accession No.
P29533
Gene ID (Entrez)
22329
Isotype
IgG2a κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The 429 monoclonal antibody reacts with mouse CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells
- Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation
- CD106 binds to integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin a4b7 (LPAM-1) and these interactions in the bone marrow and thymus are important for early lymphocyte and myeloid development
- Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response
- Applications Reported: The 429 antibody has been reported for use in flow cytometric analysis
- It has also been reported in blocking of CD106 in functional studies
- Applications Tested: The 429 antibody has been tested by flow cytometric analysis of mouse bone marrow cells
- This can be used at less than or equal to 0.25 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay
- Storage and handling: Use in a sterile environment
- Filtration: 0.2 μm post-manufacturing filtered
- VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium
- The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.