CD102 (ICAM-2) Monoclonal Antibody (3C4 (mIC2/4)), Functional Grade, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody 3C4 (mIC2/4) recognizes mouse CD102, also known as intercellular adhesion molecule-2 (ICAM-2)
• CD102, a member of the Immunoglobulin superfamily of receptors with 2 extracellular Ig domains, binds LFA (CD11a/CD18) and Mac-1 (CD11b/CD18), thereby mediating cellular interactions to lymphocytes
• CD102 plays a role in NK cytotoxicity and migration, and lymphocyte recirculation
• Expression of CD102 is found on most lymphocytes, monocytes and platelets
• The 3C4 (mIC2/4) antibody blocks interaction of lymphocytes with antigen presenting cells
• Applications Reported: This 3C4 (mIC2/4) antibody has been reported for use in flow cytometric analysis and functional assays
• Applications Tested: This 3C4 (mIC2/4) antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Storage and handling: Use in a sterile environment
• Filtration: 0.2 μm post-manufacturing filtered
• Purity: Greater than 90%, as determined by SDS-PAGE
• Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay
• Aggregation: Less than 10%, as determined by HPLC
• ICAM-2 is a member of the intercellular adhesion molecule (ICAM) family
• All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein
• ICAM-2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion
• It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance
• Several transcript variants encoding the same protein have been found for this gene
• Diseases associated with ICAM2 include Colon Carcinoma In Situ.