7091333
TOX Monoclonal Antibody (TXRX10), eFluor™ 660, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
TOX
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
1700007F02Rik; KIAA0808; thymocyte selection associated high mobility group box; thymocyte selection-associated high mobility group box; thymocyte selection-associated high mobility group box protein TOX; thymocyte selection-associated HMG box; thymus high mobility group box protein TOX; TOX; TOX1
Gene Symbols
TOX
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
TOX
Clone
TXRX10
Applications
Flow Cytometry
Conjugate
eFluor 660
Host Species
Rat
Target Species
Human, Mouse
Gene Accession No.
O94900, Q66JW3
Gene ID (Entrez)
252838, 9760
Isotype
IgG2a κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: This TXRX10 monoclonal antibody reacts with human and mouse TOX, which is also known as Thymocyte selection-associated HMG box protein
- This DNA-binding nuclear factor is expressed in thymocytes, T lymphocytes, NK cells, and lymphoid tissue-inducer (LTi) cells
- TOX expression in thymocytes is upregulated during beta-selection and positive selection
- Studies using Tox knockout mice have demonstrated a critical role for TOX in CD4+ T cell development as a regulator of the CD4loCD8lo to CD4+CD8lo transition
- Moreover, TOX has been shown to be involved in the development of NK and LTi cells
- While its regulation remains unclear, TOX expression has been shown to be induced by calcineurin-mediated TCR signaling in thymocytes during positive selection
- Applications Reported: This TXRX10 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
- Applications Tested: This TXRX10 antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcriptin Factor Buffer Set (cat 00-5523) and protocol
- Refer to Best Protocols- (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins)
- This can be used at less than or equal to 1 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- TOX (thymocyte selection-associated high mobility group (HMG) box protein) is a member of the HMG box family of DNA-binding proteins and likely plays a role in the regulation of T-cell development
- It is a 526 amino acid nuclear protein and the expression of TOX is upregulated by pre-T cell receptor (pre-TCR) and TCR activation in immature thymocytes
- TOX-mediated positive selection is associated with up-regulation of RUNX3 and is calcineurin dependent
- TOX-dependent transition to the CD4+CD8 stage is required for development of class II major histocompatibility complex-specific T cells.