Oligodendrocyte Marker O1 Monoclonal Antibody (O1), eFluor™ 660, eBioscience™, Invitrogen™

Description

• Description: This O1 monoclonal antibody reacts with galactocerebroside and other lipids found on late oligodendrocyte precursors
• O1 has been reported to react to all species tested (rat, mouse, human, chicken)
• Oligodendrocytes function as myelin-producing cells within the central nervous system ensheathing the axons of neurons with myelin in order to facilitate rapid axonal conduction
• O1 and O4 monoclonal antibodies serve as stage-specific markers of oligodendrocyte development with O4 marking early oligodendrocyte progenitors and O1-labeling late oligodendrocyte progenitors
• O4-positive cells differentiate to become O1-positive, which can in turn become mature oligodendrocytes
• Applications Reported: This O1 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, and immunocytochemistry
• Applications Tested: This O1 antibody has been tested by immunocytochemistry on fixed, differentiated OLN93 cells and can be used at less than or equal to 20 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• eFluor™ 660 is a replacement for Alexa Fluor™ 647
• eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Oligodendrocytes function as myelin-producing cells within the central nervous system ensheathing the axons of neurons with myelin in order to facilitate rapid axonal conduction
• O1 and O4 monoclonal antibodies serve as stage-specific markers of oligodendrocyte development with O4 marking early oligodendrocyte progenitors and O1-labeling late oligodendrocyte progenitors
• O4-positive cells differentiate to become O1-positive, which can in turn become mature oligodendrocytes.