7091764
IL-10 Monoclonal Antibody (JES3-9D7), PerCP-eFluor™ 710, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
IL-10
Concentration
5 μL/Test
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias
CSIF; cytokine; Cytokine synthesis inhibitory factor; GVHDS; H-IL-10; il 10; IL10; IL-10; IL-10 precursor; IL10A; IL10X; ILN; Interleukin; interleukin 10; Interleukin10; interleukin-10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor; TGIF
Gene Symbols
IL10
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
IL10
Clone
JES3-9D7
Applications
Flow Cytometry
Conjugate
PerCP-eFluor 710
Host Species
Rat
Target Species
Human
Gene Accession No.
P22301
Gene ID (Entrez)
3586
Isotype
IgG1 κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10)
- Applications Reported: This JES3-9D7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
- Applications Tested: This JES3-9D7 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Bufer Set (cat
- 88-8824) and protocol
- Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic)intracellular proteins
- This can be used at 5 μL (0.03 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5
- We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative
- Please make sure that your instrument is capable of detecting this fluorochrome
- Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
- Please protect this vial and stained samples from light
- Fixation: Samples can be stored in IC Fixation Buffer (cat
- IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes
- This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages
- It also enhances B cell survival, proliferation, and antibody production
- Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway
- Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract
- Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis
- Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.