IDO Monoclonal Antibody (mIDO-48), eFluor™ 660, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody mIDO-48 reacts with mouse indoleamine 2,3-dioxygenase 1(IDO1)
• IDO1 is an enzyme expressed intracellularly by dendritic cells, interferon gamma activated macrophages, epithelial cells, vascular endothelium and tumor cells
• This enzyme catalyzes tryptophan into kynurenine metabolites
• The reduction of tryptophan and the introduction of kynurenine metabolites in the microenvironment of IDO-expressing cells suppress T cell proliferation and promote the activation and induction of regulatory T cells
• Applications Reported: This mIDO-48 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This mIDO-48 antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (cat
• 88-8824) and protocol
• Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• IDO1 is an intracellular heme-containing enzyme that catalyzes the oxidative cleavage of the indole ring of several important regulatory molecules like tryptophan, serotonin, and melatonin
• By doing this, IDO1 initiates the production of biologically active metabolites, commonly referred to as kynurenines
• IDO1 is widely expressed in a variety of human tissues as well as in macrophages and dendritic cells (DCs)
• In inflammation, interferons (IFNs) act on specific receptors to trigger IDO1 induction
• The production of IFN-gamma and induction of IDO1 represent important antimicrobial mechanisms
• Degradation and depletion of tryptophan by IDO1 inhibits the growth of viruses, bacteria and parasites
• Furthermore, IDO1 plays a complex and crucial role in immunoregulation during infection, pregnancy, autoimmunity, transplantation, and neoplasia.