SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), eFluor™ 660, eBioscience™, Invitrogen™

Description

• Description: The eBioMC-480 (MC-480) antibody reacts with the stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine and human germ cells (EG)
• No immunoreactivity is evident with undifferentiated human EC and ES cells
• Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished
• Expression of the carbohydrate moiety is also found on mature human granulocytes (on CD15) and some monocytes
• SSEA-1 is associated with cell adhesion, migration and differentiation
• Applications Reported: This eBioMC-480 (MC-480) antibody has been reported for use in flow cytometric analysis
• Applications Tested: This eBioMC-480 (MC-480) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of the F9 cell line
• This can be used at 5 μL (0.06 μg μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• eFluor™ 660 is a replacement for Alexa Fluor™ 647
• eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g
• granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells
• There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x
• Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction
• This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells
• Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins
• CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.