7105595

GFAP Mouse, Clone: SPM248, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 2 publications

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

GFAP

Dilution

Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human, Mouse, Rat, Porcine, Bovine, Chicken, Rabbit

Gene Accession No.

P14136

Gene ID (Entrez)

2670

Immunogen

GFAP isolated from pig spinal cord

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Molecular Weight of Antigen

50 kDa

Clone

SPM248

Applications

Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Astrocyte Markers, Cancer, Cellular Markers, Cytoskeleton Markers, Neuronal Stem Cell Markers, Neuroscience, Stem Cell Markers

Formulation

PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias

FLJ45472, GFAP astrocytes, glial fibrillary acidic protein

Gene Symbols

GFAP

Isotype

IgG1

Purification Method

Protein G purified

Test Specificity

This MAb recognizes a protein of ∼50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

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Description

  • GFAP Monoclonal specifically detects GFAP in Human, Mouse, Rat, Porcine, Bovine, Chicken, Rabbit samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.