7105479

PCNA Mouse, Clone: SPM350, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

PCNA

Dilution

Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human, Mouse, Rat, Porcine, Chicken, Drosophila, Primate, Yeast, Zebrafish

Gene Accession No.

P12004

Gene ID (Entrez)

5111

Immunogen

Rat PCNA/Protein A fusion protein

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Molecular Weight of Antigen

36 kDa

Clone

SPM350

Applications

Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Autophagy, Base Excision Repair, Cell Cycle and Replication, Cellular Markers, Core ESC Like Genes, DNA Polymerases, DNA Repair, Phospho Specific, Stem Cell Markers

Formulation

PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias

cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen

Gene Symbols

PCNA

Isotype

IgG2a κ

Purification Method

Protein A purified

Test Specificity

Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.

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Description

  • PCNA Monoclonal specifically detects PCNA in Human, Mouse, Rat, Porcine, Chicken, Drosophila, Monkey, S
  • pombe, Yeast, Zebrafish samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.