7105431

alpha-Fetoprotein/AFP Mouse, Clone: C3, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 1 publication

Manufacturer: Fischer Scientific

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Antigen

alpha-Fetoprotein/AFP

Dilution

Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml, SDS-Page, Knockout Validated

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human, Porcine, Canine, Primate, Bovine (Negative), Canine (Negative), Mouse (Negative), Rat (Negative)

Gene Accession No.

P02771

Gene ID (Entrez)

174

Immunogen

Alpha fetoprotein (AFP) purified from serum of a hepatoma patient

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Molecular Weight of Antigen

70 kDa

Clone

C3

Applications

Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Cancer, GPCR, Stem Cell Markers

Formulation

PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias

Alpha-1-fetoprotein, Alpha-fetoglobulin, alpha-fetoprotein, FETA, HP, HPAFP

Gene Symbols

AFP

Isotype

IgG2a κ

Purification Method

Protein A purified

Test Specificity

It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha-fetoprotein (AFP) (ISOBM TD-2 workshop). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

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Description

  • alpha-Fetoprotein/AFP Monoclonal specifically detects alpha-Fetoprotein/AFP in Human, Porcine, Canine, Monkey, Bovine (Negative), Mouse (Negative), Rat (Negative) samples
  • It is validated for Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Flow (Intracellular), Knockout Validated.