PDGF-C, Rabbit anti-Human, Polyclonal, Millipore Sigma™
Rabbit Polyclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
PDGF-C
Applications
Western Blot
Conjugate
Unconjugated
Host Species
Rabbit
Research Discipline
Signaling
Formulation
Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Gene ID (Entrez)
NP_057289
Immunogen
A linear peptide corresponding to 21 amino acids from the C-terminal growth factor domain of human Platelet-derived growth factor C (PDGF-C).
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen
∽48 kDa observed; 39.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Dilution
Western Blotting Analysis: A representative lot detected PDGF-C in Western Blotting applications (Hurst, N.J., et. al. (2012). Biochem J. 441(3):909-18).Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected PDGF-C in HEK293T cells. (Courtesy of Hyeong-Reh Choi Kim lab at Wayne State University).
Classification
Polyclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Alias
Platelet-derived growth factor C;Fallotein;Spinal cord-derived growth factor;SCDGF;VEGF-E
Gene Symbols
PDGFC;SCDGE;UNQ174/PRO200
Isotype
IgG
Purification Method
Affinity Purified
Test Specificity
This rabbit polyclonal antibody detects full length platelet derived growth factor C (PDGF-C) and also the 18 kDa growth factor domain.
Related Products
Description
- Anti-PDGF-C, Cat
- No
- ABC1392, is a rabbit polyclonal antibody that detects Platelet-derived growth factor C and has been tested for use in Western Blotting
- Platelet-derived growth factor C (UniProt: Q9NRA1; also known as PDGF-C, Fallotein, Spinal cord-derived growth factor, SCDGF, VEGF-E) is encoded by the PDGFC (also known as SCDGE, UNQ174/PRO200) gene (Gene ID: 56034) in human
- PDGF-C is a disulfide-linked homodimeric growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis
- It also plays an important role in wound healing and angiogenesis
- It also serves as a potent mitogen and chemoattractant for cells of mesenchymal origin
- It is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off in the mature form
- Its CUB domain is localized to amino acids 46 to 163 and proteolytic removal of the CUB domain is essential for unmasking the receptor-binding epitopes of the core domain
- PDGF-C is expressed in the fallopian tube, vascular smooth muscle cells in kidney, breast and colon and in visceral smooth muscle of the gastrointestinal tract
- Highly expressed in retinal pigment epithelia
- Its high expression is also reported in retinal pigment epithelia
- In the kidney, it is constitutively expressed in parietal epithelial cells of Bowman's capsule, tubular epithelial cells, and in arterial endothelial cells
- Higher levels of PDGF-C and have been reported in the malignant breast tumor microenvironment
- In MCF-7 cells increased expression of PDGF-C is reported to enhance cell proliferation and tumor cell motility
- Tissue plasminogen activator and matriptase are shown to process PDGF-C in MCF-7 cells to its active form
- (Ref.: Hurst, NJ., et al
- (2012)
- Biochem
- J
- 441(3); 909-918; Lei, H., and Kazlauskas, A (2008)
- Exp
- Eye Res
- 86(5); 711-712).