Neurogranin, Rabbit anti-Rat, Human, Polyclonal, Millipore Sigma™
Rabbit Polyclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Neurogranin
Applications
Immunohistochemistry (Paraffin), Western Blot
Conjugate
Unconjugated
Regulatory Status
RUO
Target Species
Rat, Human
Gene Alias
Ng;RC3
Gene Symbols
NRGN
Isotype
IgG
Purification Method
Affinity Purified
Test Specificity
This rabbit polyclonal antibody sepecifically detects Neurogranin. It targets an epitope with in 12 amino acids from the C-terminal region.
Dilution
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Neurogranin in human and rat brain tissue sections.
Classification
Polyclonal
Host Species
Rabbit
Research Discipline
Neuroscience
Formulation
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_001119653
Immunogen
KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of human Neurogranin.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Molecular Weight of Antigen
∽15 kDa observed; 7.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Related Products
Description
- Anti-Neurogranin Antibody, Cat
- No
- 07-425-I, is a highly specific rabbit polyclonal antibody that targets Neurogranin and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting
- Neurogranin (UniProt: Q92686; also known as Ng, RC3) is encoded by the NRGN gene (Gene ID: 4900) in human
- Neurogranin is abundantly expressed in cerebral cortex, hippocampus, amygdala, and striatum
- In neurons, it is found concentrated at dendritic spines
- It acts as a third messenger and serves as a substrate for protein kinase C-mediated molecular cascades during synaptic development and remodeling
- It contains an IQ domain (aa 26-347) and a collagen-like domain (aa 48-78)
- The IQ domain mediates its interaction with calmodulin and phosphatidic acid (PA)
- Neurogranin is intrinsically unstructured, however, upon binding with calmodulin, the IQ domain adopts a helical conformation
- Neurogranin is phosphorylated at serine 36 by protein kinase C and following phosphorylation neurogranin is unable to bind either calmodulin or PA, which can lead to decline in memory-associated functions
- Higher levels of neurogranin have also been reported in brains of elderly and is considered a factor in memory decline, independent of Alzheimer s disease
- (Ref.: Javier Dı´ez-Guerra, F
- (2010)
- Life
- 62(8): 597-606; Casaletto, KB et al
- (2017)
- Neurology 89(17); 1782-1788).