Rhodopsin, Rabbit anti-Human,Mouse,Rat,Amphibian,Bovine,Zebrafish, Clone: Rho 1D4, Novus Biologicals™
Rabbit Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Rhodopsin
Concentration
1 mg/mL
Applications
Western Blot, ELISA, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Radioimmune Assays (RIA)
Conjugate
Unconjugated
Host Species
Rabbit
Research Discipline
GPCR, Phospho Specific, Signal Transduction, Vision
Formulation
PBS
Gene ID (Entrez)
6010
Immunogen
Bleached bovine rod outer segment (ROS).
Primary or Secondary
Primary
Content And Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Clone
Rho 1D4
Dilution
Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Mouse, Rat, Amphibian, Bovine, Zebrafish
Gene Alias
CSNBAD1, MGC138309, OPN2MGC138311, opsin 2, rod pigment, opsin-2, retinitis pigmentosa 4, autosomal dominant, rhodopsin, RP4
Gene Symbols
RHO
Isotype
IgG κ
Purification Method
Protein A purified
Test Specificity
The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.
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Description
- Rhodopsin Monoclonal specifically detects Rhodopsin in Human, Mouse, Rat, Amphibian, Bovine, Zebrafish samples
- It is validated for Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay.