TNF alpha Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™
Rabbit Polyclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
TNF alpha
Applications
ELISA, Western Blot
Conjugate
Biotin
Host Species
Rabbit
Target Species
Rat
Gene Accession No.
P16599
Gene ID (Entrez)
24835
Immunogen
E.coli-derived, 17.3 kDa Recombinant Rat TNF-alpha
Purification Method
Antigen affinity chromatography
Gene
Tnf
Concentration
0.1-1.0 mg/mL
Classification
Polyclonal
Form
Lyophilized
Regulatory Status
RUO
Formulation
PBS with no preservative
Gene Alias
APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
Gene Symbols
Tnf
Primary or Secondary
Primary
Content And Storage
-20°C
Related Products
Description
- AA Sequence of recombinant protein: MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVFQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL
- Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat TNF-alpha
- Anti-Rat TNF-alpha-specific antibody was purified by affinity chromatography and then biotinylated
- Sandwich ELISA: To detect Rat TNF-alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
- This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat TNF-alpha (500-P72) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat TNF-alpha
- Western Blot: To detect Rat TNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
- When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Rat TNF-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
- 500-P72BT-1MG will be provided as 2 x 500 μg TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily
- This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR
- TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation
- TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions
- Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice
- Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors
- TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity
- This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.