SCF Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™
Rabbit Polyclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
SCF
Applications
ELISA, Western Blot
Conjugate
Biotin
Host Species
Rabbit
Target Species
Human
Gene Accession No.
P21583
Gene ID (Entrez)
4254
Immunogen
E.coli-derived, 18.4 kDa Recombinant Human SCF
Purification Method
Antigen affinity chromatography
Gene
KITLG
Concentration
0.1-1.0 mg/mL
Classification
Polyclonal
Form
Lyophilized
Regulatory Status
RUO
Formulation
PBS with no preservative
Gene Alias
710-712; blz; c-Kit ligand; Clo; cloud gray; Con; contrasted; CSF; DCUA; DFNA69; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; FPHH; Gb; grizzle-belly; hematopoietic growth factor KL; H-SCF; Kit ligand; Kitl; Kitlg; kit-ligand; KL-1; Mast cell growth factor; MGF; M-SCF; Processed kit ligand; SCF; SF; SHEP7; sKITLG; Sl; SLF; Soluble KIT ligand; Steel factor; steel factor/kit ligand; stem cell factor; stem cell factor KL-1; stem cell factor, CSF
Gene Symbols
KITLG
Primary or Secondary
Primary
Content And Storage
-20°C
Related Products
Description
- AA Sequence of recombinant protein: MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA
- Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human SCF
- Anti-Human SCF-specific antibody was purified by affinity chromatography and then biotinylated
- Sandwich ELISA: To detect hSCF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
- This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human SCF (500-P48) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hSCF
- Western Blot: To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
- Used in conjunction with compatible secondary reagents the detection limit for Recombinant hSCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
- 500-P48BT-1MG will be provided as 2 x 500 μg Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways
- Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways
- SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines
- The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma
- At least two isoforms of SCF are known to exist.