CXCL1 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™
Rabbit Polyclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CXCL1
Applications
ELISA, Western Blot
Conjugate
Biotin
Host Species
Rabbit
Target Species
Human
Gene Accession No.
P09341
Gene ID (Entrez)
2919
Immunogen
E.coli-derived Recombinant Human GRO-alpha/MGSA (CXCL1)
Purification Method
Antigen affinity chromatography
Gene
Cxcl1
Concentration
0.1-1.0 mg/mL
Classification
Polyclonal
Form
Lyophilized
Regulatory Status
RUO
Formulation
PBS with no preservative
Gene Alias
alpha-chemokine; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 1; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); Cinc1; CINC-1; CXC; CXC motif chemokine; C-X-C motif chemokine 1; C-X-C motif chemokine ligand 1; CXCL; CXCL1; CXCL-1; Cytokine-induced neutrophil chemoattractant 1; fibroblast secretory protein; Fsp; Gro; GRO a; GRO a; Gro1; GRO1 oncogene; GRO1 oncogene (melanoma growth stimulating activity, alpha); GRO1 oncogene (melanoma growth-stimulating activity); GROa; GROalpha; GRO-alpha(1-73); GRO-alpha(4-73); GRO-alpha(5-73); GRO-alpha(6-73); Growth-regulated alpha protein; GROa; Hematopoietic synergistic factor; HSF; KC; KC(5-72); KC/GR)-alpha; KC/GRO-alpha; KC-T; melanoma growth stimulating activity, alpha; Melanoma growth stimulatory activity; melanoma growth stimulatory activity alpha; MGSA; MGSA alpha; MGSA-a; N51; NAP3; NAP-3; Neutrophil-activating protein 3; platelet-derived g
Gene Symbols
Cxcl1
Primary or Secondary
Primary
Content And Storage
-20°C
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Description
- AA Sequence of recombinant protein: ASVATELRCQ CLQTLQGIHP KNIQSVNVKS PGPHCAQTEV IATLKNGRKA CLNPASPIVK KIIEKMLNSD KSN
- Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human GRO-alpha/MGSA (CXCL1)
- Anti-Human GRO-alpha/MGSA (CXCL1)-specific antibody was purified by affinity chromatography and then biotinylated
- Sandwich ELISA: To detect Human GRO-alpha/MGSA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
- This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human GRO-alpha/MGSA (500-P92) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human GRO-alpha/MGSA
- Western Blot: To detect Human GRO-alpha/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
- Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human GRO-alpha/MGSA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
- 500-P92BT-1MG will be provided as 2 x 500 μg CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines
- Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators
- In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.