MilliporeSigma™ VprBP, Rabbit, Unlabeled, Polyclonal,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | ABS1583-Each-of-1 | In Stock | ₹ 40,228.00 |
ABS1583 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 40,228.00
GST (18%): ₹ 7,241.04
Total Price: ₹ 47,469.04
Antigen
VprBP
Classification
Polyclonal
Conjugate
Unconjugated
Gene
VPRBP, DCAF1, KIAA0800, RIP
Host Species
Rabbit
Purification Method
Affinity Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Specificity was verified by reduced target band detection upon shRNA-mediated VprBP knockdown in U2OS, WI-38/E6, HeLa, and HEK293T cells, as well as following cre expression in MEFs harboring floxed Vprbp alleles (McCall, C.M., et al. (2008). Mol. Cell. Biol. 28(18):5621-5633; Nakagawa, T., et al. (2015). 57(2):247-260). Target region is present in human spliced isoforms 1-4 as well as mouse isoforms 1 and 2 reported by UniProt (Q9Y4B6, Q80TR8), but missing in mouse isoforms 3 and 4 and not present in rat VprBP (NM_001191798.1 & NP_001178727.1).
Content And Storage
Stable for 1 year at 2°-8°C from date of receipt.
Applications
Western Blot
Concentration
Please refer to lot specific datasheet.
Formulation
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.
Q9Y4B6
Immunogen
KLH-conjugated linear peptide corresponding to human VprBP C-terminal end sequence.
Quantity
100 μL
Research Discipline
Signaling
Gene ID (Entrez)
NP_055518
Target Species
Human
Related Products
Description
- Protein VPRBP (EC 2.7.11.1; UniProt Q9Y4B6; also known as DDB1- and CUL4-associated factor 1, HIV-1 Vpr-binding protein, Serine/threonine-protein kinase VPRBP, Vpr-interacting protein, VprBP) is encoded by the VPRBP (also known as DCAF1, KIAA0800, RIP) gene (Gene ID 9730) in human
- VprBP/DCAF1 acts as an adaptor molecule that mediates substrates recruitment for both the RING-type CRL4 (Cul4-Roc1-DDB1) and HECT-type EDD/UBR5 E3 ubiquitin ligase complexes
- CRL4 substrates known to be recruited by VprBP include Merlin, Mcm10, UNG2, SMUG1, RORαme1
- Known EDD/UBR5 substrates recruited by VprBP include Katanin and TERT
- VprBP is involved in a variety of normal cellular processes, including proliferation, DNA replication, cell cycle progression, telomere maintenance, and DNA damage responses
- All three TET methylcytosine dioxygenases are targeted by CRL4-VprBP for monouquitination on a highly conserved lysine residue (human TET1 K1589, TET2 K1299, and TET3 K859), an essential event for TET chromatin binding, Vprbp gene knockout in mouse oocytes abrogates paternal DNA hydroxymethylation in zygotes, and the monoubiquitylation site is among multiple recurrent TET2-inactivating mutations targeted by leukemia
- As indicated by its name, VprBP is also a well known target utilized by the Vpr and Vpx accessory factors of primate lentiviruses to highjack the host CRL4-VprBP E3 ligase function.