MilliporeSigma™ phospho LSD1 (Ser112), Rabbit, Unlabeled, Polyclonal,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | ABE1462MI-Each-of-1 | In Stock | ₹ 40,292.08 |
ABE1462MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 40,292.08
GST (18%): ₹ 7,252.574
Total Price: ₹ 47,544.654
Antigen
phospho LSD1 (Ser112)
Classification
Polyclonal
Conjugate
Unconjugated
Gene
Kdm1a, Aof2, Kiaa0601, Lsd1
Host Species
Rabbit
Purification Method
Affinity Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
The Ser112 designation is based on murine KDM1A/LSD1 sequence (UniProt Q6ZQ88). Murine Ser112 is equivalent to human Ser111 and rat Ser106 (UniProt O60341 & F1MA31). Specificity of this polyclonal antibody was confirmed by dot blot analysis using the immunogen peptide and the corresponding non-phosphorylated peptide, as well as by Western blotting analysis of PMA-induced target phosphorylation, lambda phosphatase treatment of the cell lysate prior to Western blotting completely abolished the target band detection (Nam, H.J., et al. (2014). Mol. Cell 53:791-805).
Content And Storage
Stable for 1 year at 2°-8°C from date of receipt.
Applications
Western Blot
Concentration
Please refer to lot specific datasheet.
Formulation
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.
Q6ZQ88
Immunogen
Linear peptide corresponding to a mouse LSD1 sequence containing the phosphorylated Ser112.
Quantity
100 μL
Research Discipline
Epigenetics & Nuclear Function
Gene ID (Entrez)
NP_598633
Target Species
Human
Related Products
Description
- Lysine-specific histone demethylase 1A (UniProt Q6ZQ88; also known as BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2) is encoded by the Kdm1a (also known as Aof2, Kiaa0601, Lsd1) gene (Gene ID 99982) in murine species
- The FAD+-dependent, lysine-specific demethylase (LSD) LSD1 is a component of the REST corepressor (CoREST) complexes that mediate gene repression by catalyzing the demethylation of mono- and di-methylated histone H3 Lys4 (H3K4me1/2)
- LSD1 is also involved in gene activation process associated with nuclear receptors through demethylation of H3K9me1/2
- In addition, LSD1 is known to demethylate non-histone substrates, such as p53 and Dnmt1
- LSD1 is reported to be phosphorylated on Ser112 by PKCalpha and thereby mediate PKCalpha signaling-dependent circadian clock regulation
- Ser112 phosphorylation induces LSD1 interaction with CLOCK:BMAL1 to facilitate E-box-dependent transcriptional activation
- Knockin mice bearing Lsd1(SA/SA) alleles coding for phosphorylation-defective S112A LSD1 mutant are reported to exhibit altered circadian rhythms in locomotor behavior with attenuated rhythmic expression of core clock genes and impaired circadian clock phase resetting.