MilliporeSigma™ anti-beta Actin, Polyclonal
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | ABT264MI-Each-of-1 | In Stock | ₹ 47,205.60 |
ABT264MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 47,205.60
GST (18%): ₹ 8,497.008
Total Price: ₹ 55,702.608
Antigen
beta Actin
Classification
Polyclonal
Formulation
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Gene Symbols
ACTB
Immunogen
C-terminally KLH-conjugated linear peptide corresponding to human beta-actin N-terminal sequence with arginylation modification.
Quantity
100 μg
Research Discipline
Cell Structure
Gene ID (Entrez)
NP_001092
Target Species
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot
Conjugate
Unconjugated
Gene Accession No.
P60709
Host Species
Rabbit
Purification Method
Affinity Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
This polyclonal antibody detects beta-actin posttranslationally modified by the removal of Met1 and Asp 2 and the arginylation of Asp3. This antibody does not react with beta-actin with unmodified N-terminus.
Content And Storage
2°C to 8°C for one year
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Description
- Specifically detects beta Actin Clone: in Human, Mouse, Rat samples, and it is validated for Immunocytochemistry, Western Blotting Actin, cytoplasmic 1 (UniProt P60709; also known as beta-actin, PS1TP5-binding protein 1) is encoded by the ACTB (also known as BRWS1, PS1TP5BP1) gene (Gene ID 60) in human
- Post-translational protein arginylation is mediated by Arg–transfer RNA (-tRNA) protein transferase (Ate1; arginyltransferase).β- andγ-actins are the major structural components of the actin cytoskeleton in nonmuscle cells
- Despite their similarity, theβ-, but not theγ-, actin is N-terminally arginylated in vivo, where the first two N-terminal amino acids are removed and replaced with an arginine (from NH2-MDDDIAAL- to NH2-RDDIAAL-)
- In the absence of arginylation, actin forms aggregates during cell-free polymerization
- Fibroblasts derived from Ate1 knockout (KO) mice show collapsed lamella, which can be rescued by reintroducing N-terminally arginylatedβ-actin into the Ate1 KO cells
- Intracellular actin polymer levels are shown to drastically decrease in arginylation-deficient cells, which results from changes in actin polymerization properties.