HIF-1 beta Polyclonal Antibody, Invitrogen™

Description

• In Western blot, background may increase with whole cell extract preparations
• Lower dilutions may be needed for whole cell extract preps
• Suggested positive control: MCF-7 human nuclear extracts, MCF-7 whole cell lysate, antigen standard for ARNT (transient overexpression lysate)
• HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products
• Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease
• Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease
• HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis
• This occurs as a posttranslational modification by prolyl hydroxylation
• HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits
• Both subunits are constantly translated
• However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes
• Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity
• Therefore, the concentration of HIF-1 alpha increases in the cell
• In contrast, HIF-1 beta remains stable under either condition
• HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products
• Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products
• Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease
• Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease
• HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis
• This occurs as a posttranslational modification by prolyl hydroxylation
• HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits
• Both subunits are constantly translated
• However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes
• Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity
• Therefore, the concentration of HIF-1 alpha increases in the cell
• In contrast, HIF-1 beta remains stable under either condition
• HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products
• Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.