IKKβ w/control Mouse anti-Human, Unlabeled, Clone: F18-1875, BD

Description

• HeLa Control Lysate
• Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses
• The nuclear transcription factor kappa-B, NF-κB, is controlled by interaction with an inhibitory subunit, I-κB, which restricts NF-κB to the cytoplasm
• Following stimulation by various cytokines or other stimuli, I-κB becomes degraded and NF-κB is released to the nucleus
• The release of I-κB from NF-κB is thought to be a critical point in the activation of NF-κB signal pathways
• A group of proteins form an NF-κB regulatory complex, or signalsome
• Two members of this complex are a pair of closely related serine/threonine kinases, IKKα and IKKβ (also called IKK-1 and IKK-2), which phosphorylate critical residues of I-κB, thus targeting it for subsequent degradation
• The IKK complex contains similar amounts of IKKα and IKKβ, as well as two other polypeptides, which are differentially processed forms of a third subunit, IKKγ
• IKKα and IKKβ become activated following phosphorylation by upstream kinases, including NF-κB-inducing kinase (NIK) and MEKK1
• IKKγ interacts preferentially with IKKβ and is required for the activation of the IKK complex
• Experiments done on mice lacking IKKβ demonstrate that these mice died at mid-gestation from uncontrolled liver apoptosis
• Furthermore, when cells from IKKβ-deficient m ice were treated with tumor necrosis factor α or interleukin 1, neither IKK nor NF-κB were activated
• Thus IKKβ, but not IKKα, plays a pivotal role in the induction of NF-κB and activation of IKK
• IKKβ migrates at ∽87kDa in SDS/PAGE
• The antibody recognizes human IKKβ
• A recombinant protein corresponding to amino acids 1-666 of human IKKβ was used as the immunogen
• Host Species: Mouse Clone: F18-1875 Isotype: IgG1 κ Species Reactivity: Human Immunogen: Human IKKβ recombinant protein aa
• 1-666 Western Blotting