Retinoblastoma Protein Mouse anti-Human, Unlabeled, Clone: G3-245, BD

Description

• Members of the retinoblastoma (Rb) family, including the related proteins p107 and p130, share several properties, including the ability to regulate E2Fdependent transcription and to regulate cell-cycle progression
• The Rb gene product is a phosphoprotein that is expressed in most normal cells of vertebrates
• Rb acts as a tumor suppressor by providing a cell cycle checkpoint between the G1 and S phases
• The active, underphosphorylated form of Rb (Rb or pRb) is primarily found in resting or fully differentiated cells
• The activity of Rb is negatively regulated by cyclin dependent kinases, which phosphorylate Rb in late G1
• Thus, the hyperphosphorylated form (ppRb) is primarily found in proliferating cells
• pRB inactivation is a critical step leading to S-phase commitment at the G1 checkpoint of the cell cycle
• In addition, the underphosphorylated form of Rb may bind to viral oncogenes such as SV40 large T Ag, adenoviral EIA and HPV-E7, which may contribute to the transforming activity of these viral oncoproteins
• G3-245 was made using a Trp-E-Rb fusion protein as immunogen and recognizes an epitope between amino acids 332-344 (DARLFDHDKTLQ) of the human retinoblastoma protein (pp110-114 Rb)
• In western blot analysis, Rb migrates as multiple closely-spaced bands between 110-116kda on SDS-PAGE
• The bands represent different Rb phosphorylation states where the level of Rb phosphorylation can be cell cycle and/or cell-type dependent (i.e all forms may not be seen in all cell types that express Rb)
• G3-245 has been reported to recognize human, monkey, mouse, rat, mink and a putative quail Rb
• This antibody has also been referred to as Mh-RB-02,20 and mAb-245
• Host Species: Mouse Clone: G3-245 Isotype: IgG1 Species Reactivity: Human Immunogen: Human Rb aa
• 332-344 Formula Weight [Chemical]: 110-116kDa Bioimaging, Blocking Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunoprecipitation, Intracellular Staining, Western Blotting