p53 Mouse anti-Human, Unlabeled, Clone: DO-1, BD

Description

• The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers
• Missense mutations occur in tumors of the colon, lung, breast, ovary, bladder and several other organs
• The mutant p53 is overexpressed in a variety of transformed cells and wild-type p53 forms specific complexes with several viral oncogenes including SV40 large T, E1B from adenovirus, and E6 from human papilloma virus
• Wild type p53 plays a role as a checkpoint protein for DNA damage during the G1/S-phase of the cell cycle
• However, it is still unclear, whether point mutated forms of p53 are simple null mutants and/or dominant negatively acting proteins
• DO-1 reacts with human wild-type and mutant p53
• It cross-reacts with bovine p53 but does not cross-react with mouse or rat p53
• DO-1 recognizes an epitope between amino acids 1 and 45 of all known forms of human p53
• Human recombinant p53 protein was used as immunogen
• Host Species: Mouse Clone: DO-1 Isotype: IgG2a Species Reactivity: Human Immunogen: Human p53 Formula Weight [Chemical]: 53kDa Immunohistochemistry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blotting