PTEN Mouse, Unlabeled, Clone: A2B1, BD

Description

• Cancer can develop when cells escape normal growth control mechanisms through mutations in proto-oncogenes or tumor suppressor genes
• A characteristic of most oncogene and tumor suppressor gene products is that they are components of signal transduction pathways that are essential for maintaining cellular homeostasis
• PTEN (phosphatase and tensin homolog detected on chromosome ten/MMAC or TEP1 (mutated in multiple advanced cancers) is a tumor suppressor gene that has been identified as mutated in multiple tumor types
• PTEN is a phosphatase that acts on proteins and lipid second messengers
• The ability of PTEN to cause tumors is thought to result from the loss of its negative control on the phosphoinositide 3-kinase signaling pathway which regulates cell growth and survival
• Using a mutant mouse model for PTEN, Suzuki et al
• demonstrated that these mice had elevated levels of the protein kinase Akt in tumors associated with loss of heterozygosity of mPTEN
• Human PTEN is a polypeptide of 403 amino acids based on the nucleotide sequence and is observed to migrate at ∽55kDa in SDS/PAGE
• The antibody recognizes human and mouse PTEN
• A peptide representing amino acids 387-400 at the C-terminus of human PTEN was used as the immunogen
• Western Blotting