CD193 Mouse anti-Human, Brilliant Violet 421, Clone: 5E8, BD

Description

• The 5E8 monoclonal antibody specifically binds to human CCR3 which is also known as CD193
• CCR3 is a G protein-linked, 7 transmembrane, chemokine receptor expressed on a variety of hematopoietic cells
• Similar to CCR5 and CXCR4, CCR3 can be a co-receptor for HIV-1
• It is primarily expressed by eosinophils and basophils during atopic conditions, dermatitis, allergic rhinitis, conjunctivitis and bronchial asthma
• Chemokines including RANTES, Eotaxin, MCP-3, MIP1 α have been reported to act as ligands for CCR3 and stimulate CCR3+ cells
• Eotaxin stimulates Th2 cells expressing CCR3
• Other studies describe HIV-1 specific T cell cytotoxicity can be mediated by RANTES and Eotaxin through CCR3
• CCR3 expressed on dendritic cells may have a biological role on cell-cell interaction during antigen presentation
• CCR3 has been clustered as CD193 in the HLDA VIIIth workshop
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter)
• BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.