CD140a Mouse anti-Human, Brilliant Violet 421, Clone: αR1, BD

Description

• The αR1 monoclonal antibody specifically binds to the human platelet derived growth factor (PDGF) receptor α (PDGFRα), also known as CD140a
• CD140a is a 170 kDa single transmembrane glycoprotein expressed on fibroblasts, smooth muscle cells, glial cells and chondrocytes
• PDGF receptors α and β are single glycoproteins with intracellular tyrosine kinase domains
• They are structurally similar to the M-CSF receptor and CD117 (c-kit)
• Their ligand, PDGF, is a mitogen for connective tissue cells and glial cells
• PDGF plays a role in wound healing and it also acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils
• Functional PDGF is secreted in disulfide linked, homodimeric or heterodimeric forms comprised of A or B chains (PDGFAA, PDGF-BB or PDGF-AB)
• Binding of divalent PDGF induces receptor dimerization with three possible forms: αα, αβ, ββ
• The PDGFRα subunit binds both PDGF A and B chains, whereas the PDGFRβ subunit binds only PDGF B chains
• Although both receptor subunits can stimulate mitogenic responses, only the β subunit can induce chemotaxis
• The αR1 antibody is specific for PDGFRα and does not crossreact with PDGFR β
• It immunoprecipitates human, monkey, rabbit, pig, dog and cat PDGFR α
• It does not recognize hamster, rat or mouse PDGFR α
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter)
• BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.