CD127 Rat anti-Mouse, Brilliant Violet 421, Clone: SB/199, BD

Description

• The SB/199 monoclonal antibody specifically binds to mouse CD127, the 65-75 kDa type-I transmembrane protein IL-7Rα
• The high affinity IL-7 receptor complex is composed of at least two transmembrane proteins, IL-7Rα and CD132, the common γ chain
• CD127 has some sequence homology to the cytokine receptor superfamily (also known as the hematopoietin receptor superfamily)
• Micelacking CD127 display profoundly impaired development of the B and T lymphoid cell lineages, but display no obvious non-lymphoid abnormalities
• IL-7Rα is expressed on common lymphoid progenitors and early stages of B lineage development in the bone marrow, on the earliest thymocyte progenitors, on CD4-CD8- double-negative and CD4+ and CD8+ single-positive thymocytes, and on most peripheral T lymphocytes
• Intestinal intraepithelial lymphocytes with low-density γδ TCR upregulate CD127 expression in response to IL-2, which may be secreted by neighboring αβ TCR-bearing T cells
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter)
• BD Horizon™ BV421conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.