CD127 Rat anti-Mouse, Brilliant Violet 786, Clone: SB/199, BD

Description

• The SB/199 monoclonal antibody specifically binds to mouse CD127, the 65-75 kDa type-I transmembrane protein IL-7R α
• The high affinity IL-7 receptor complex is composed of at least two transmembrane proteins, IL-7R α and CD132, the common γchain
• CD127 has some sequence homology to the cytokine receptor superfamily (also known as the hematopoietin receptor superfamily)
• Mice lacking CD127 display profoundly impaired development of the B and T lymphoid cell lineages, but display no obvious non-lymphoid abnormalities
• IL-7R α is expressed on common lymphoid progenitors and early stages of B lineage development in the bone marrow, on the earliest thymocyte progenitors, on CD4-CD8- double-negative and CD4+ and CD8+ single-positive thymocytes, and on most peripheral T lymphocytes
• Intestinal intraepithelial lymphocytes with low-density γδ TCR upregulate CD127 expression in response to IL-2, which may be secreted by neighboring αβ TCR-bearing T cells
• The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm
• BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60-nm filter).