IL-17A Mouse anti-Human, Brilliant Violet 786, Clone: N49-653, BD

Description

• Human IL-17A, also known as IL-17, is a proinflammatory cytokine that is encoded by the IL17A gene in chromosome 6
• IL-17A is produced as a disulfide-linked homodimer comprised of two mature 136-amino acid polypeptides
• It is a member of the IL-17 family of structurally related cytokines, designated IL-17A through IL-17F
• Activated memory T cells, especially Th17 cells (specialized IL-17A-producing CD4+ T cells distinct from Th1 and Th2 cells) produce IL-17 and provide protective immunity against pathogens
• Activated CD8+ T cells, γδT cells, NK cells and neutrophils can also be activated to produce IL-17A
• IL-17A binds to and exerts its biological activity through IL-17 receptors (IL-17R) that are expressed by a variety of target cells including fibroblasts, epithelial and endothelial cells, monocytes/macrophages and mast cells
• The ubiquitous IL-17R expression pattern may explain the broad tissue responsiveness to IL-17
• IL-17 induces stromal cells to secrete cytokines and chemokines involved in inflammatory and hematopoietic processes
• For example, IL-17 induces fibroblasts to produce IL-6, IL-8, G-CSF and express increased surface ICAM-1
• The N49-653 antibody reacts with human IL-17A
• The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm
• BD Horizon™ BV786 can be excited by the violet laser and detected in a filter used to detect Cy7™-like dyes (eg, 780/60-nm filter).