CD273 Mouse anti-Human, Brilliant Violet 650, Clone: MIH18, BD

Description

• The MIH18 monoclonal antibody specifically binds to CD273 which is also known as the Programmed Death Ligand 2 (PD-L2) and B7-DC
• CD273 is a type 1 transmembrane glycoprotein member of the B7 family and Ig superfamily
• CD273 serves as a ligand for CD279, the Programmed Death 1 (PD-1) receptor
• CD273 is expressed by dendritic cells, activated monocytes, medullary thymic epithelial cells, placental trophoblasts, and myocardial endothelium
• CD273 can function as a coinhibitor of T cell functions by acting through CD279
• Monoclonal antibodies that block the CD273 and CD279 interaction result in enhanced T cell proliferation and cytokine production
• Thus, CD273 can play an important role in regulating T cell responses
• Clone MIH18 is reportedly a blocking antibody
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will bespillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.