CD25 Mouse anti-Human, Brilliant Violet 421, Clone: 2A3, BD

Description

• The 2A3 monoclonal antibody specifically binds to human CD25, the low-affinity alpha subunit of the Interleukin-2 Receptor (IL-2R α)
• CD25 associates with CD122 (IL-2R β chain) and CD132 (common γchain or γc) to form the high-affinity signal-transducing IL-2R complex
• CD25 is expressed by a subset of peripheral blood lymphocytes including CD4+CD25+ natural regulatory T cells
• CD25 antigen density increases on activated T cells including phytohemagglutinin (PHA)-, concanavalin A (Con A)-, and CD3-activated T lymphocytes
• High levels of CD25 can also be expressed by T lymphocytes from mixed lymphocyte cultures and by human T-lymphocyte leukemia virus (HTLV)-infected T-lymphocyte leukemia lines, for example, HUT-102
• Recombinant IL-2 blocks the binding of the 2A3 antibody to PHA-activated T lymphocytes
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter)
• BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates.