CD47 Mouse anti-Human, Brilliant Violet 421, Clone: B6H12, BD

Description

• The B6H12 monoclonal antibody specifically binds to CD47, a 42-52 kDa N-linked glycan protein
• CD47 antigen, also known as integrin-associated protein (IAP), is expressed on all hematopoietic cells, including leukocytes, platelets and erythrocytes
• It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines
• CD47 may play a role as a signal transducer in the regulation of cation fluxes across cell membranes and in the chemotactic and adhesive interactions of leukocytes with endothelial cells
• The B6H12 antibody is capable of blocking phagocytosis of microparticles by peripheral blood granulocytes
• It has also been reported to induce proliferation of CD3-activated T cells The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon™ BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon™ BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.