CD3 Rat anti-Murine, Brilliant Ultraviolet 737, Clone: 17A2, BD Horizon™

Description

• The 17A2 monoclonal antibody specifically binds to the T-cell receptor-associated CD3 complex that is expressed on many thymocytes and mature T lymphocytes
• Plate-bound 17A2 antibody has been reported to induce IL-2 production by cultured T cells in the absence of accessory cells
• The binding of the 17A2 antibody to T cells can be blocked by the anti-CD3e mAb 145-2C11
• This suggests that the 17A2 antibody recognizes an epitope of the CD3 epsilon chain
• In vivo treatment with 17A2 antibody has been reported to partially deplete T lymphocytes and temporarily down-modulates CD3 expression on T cells
• The antibody was conjugated to BD Horizon BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm
• BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter
• Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter).