MA181819
Nestin Monoclonal Antibody (Rat-401 (4D4)), Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MA181819-Each-of-1 | In Stock | ₹ 87,264.50 |
MA181819 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 87,264.50
GST (18%): ₹ 15,707.61
Total Price: ₹ 1,02,972.11
Antigen
Nestin
Classification
Monoclonal
Concentration
1 mg/mL
Formulation
TBS with 0.05% sodium azide
Gene Accession No.
P21263, Q6P5H2
Gene Symbols
Nes
Immunogen
Nestin purified from embryonic rat spinal cord.
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Mouse, Rat
Product Type
Antibody
Isotype
IgG1
Applications
Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
Clone
Rat-401 (4D4)
Conjugate
Unconjugated
Gene
Nes
Gene Alias
AA166324; C78523; ESTM46; FLJ21841; intermediate filament protein; Nbla00170; Nes; Nestin
Host Species
Mouse
Purification Method
Protein A
Regulatory Status
RUO
Gene ID (Entrez)
18008, 25491
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Form
Liquid
Related Products
Description
- This antibody does not react with human
- In Western blot, this antibody detects a ∽200-220 kDa band in newborn rat or mouse cell extracts
- For immunohistochemistry, we recommend perfusing tissues with 4% paraformaldehyde (pH 7.4) for light microscopy and with either 4% paraformaldehyde (pH 10.0) or 4% paraformaldehyde with 0.1% glutaraldehyde (pH 7.4) for EM
- For Immunocytochemistry we recommend using cells fixed in 4% paraformaldehyde buffered with 50mM sodium borate (pH 9.5)
- For Western blot, it is recommended that samples should be boiled in 4 volumes of 125 mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol and 4.6% SDS
- Membranes should be blocked with milk or BSA
- 5% PAGE gels are suggested
- Mouse anti Rat Nestin antibody, clone Rat-401 recognizes rat nestin, a large intermediate filament protein transiently expressed in embryonic glian cells (Hockfield and McKay 1985)
- Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells
- Nestin has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells
- Also, Nestin is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors
- Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells
- Nestin expression has been observed in pancreatic hematopoietic stem cell populations, and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.