Importin beta-1 Monoclonal Antibody (3E9), Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MA3070-Each-of-1 | In Stock | ₹ 48,282.50 |
MA3070 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 48,282.50
GST (18%): ₹ 8,690.85
Total Price: ₹ 56,973.35
Antigen
Importin beta-1
Classification
Monoclonal
Concentration
1 mg/mL
Formulation
PBS with 0.05% sodium azide
Gene Accession No.
P52296, P70168, Q14974
Gene Symbols
KPNB1
Immunogen
Purified NTF97 from bovine erythrocytes.
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Bovine, Canine, Human, Mouse, Porcine, Rat
Product Type
Antibody
Isotype
IgG2a
Applications
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Inhibition Assays, Immunoprecipitation, Western Blot
Clone
3E9
Conjugate
Unconjugated
Gene
KPNB1
Gene Alias
AA409963; HGNC:6400; IMB1; Impnb; importin 1; importin 90; importin beta; importin beta-1 subunit; importin subunit beta-1; Importin-90; IPO1; IPOB; KapB1; karyopherin (importin) beta 1; karyopherin beta 1; karyopherin subunit beta 1; karyopherin subunit beta-1; karyopherin,beta 1; Kpnb1; MGC2155; MGC2156; MGC2157; NTF97; Nuclear factor p97; pore targeting complex 97 kDa subunit; PTAC97; SCG
Host Species
Mouse
Purification Method
Protein A
Regulatory Status
RUO
Gene ID (Entrez)
100518449, 16211, 24917, 3837, 491042, 507804
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Form
Liquid
Related Products
Description
- MA3-070 detects Importin beta 1 from human, canine, bovine, rat and mouse tissues as well as porcine cell lysates
- Importin beta 1 is also known as NTF97, Nuclear Transport Factor p97, and karyopherin beta 1
- This antibody does not detect bacterially expressed recombinant NTF97
- MA3-070 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation procedures
- By Western blot, this antibody detects a single ∽97 kDa protein representing NTF97 in MDBK cell homogenate
- Immunofluorescence staining of NTF97 in MDBK cells with MA3-070 results in the labeling of both the nuclear envelope (NE) and the cytoplasm and shows discontinuous, punctate staining along the rim of the NE
- This antibody can inhibit import of nuclear localization sequence-containing proteins in permeabilized cells
- The MA3-070 antigen is purified NTF97 from bovine erythrocytes
- Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope
- The import of proteins containing a nuclear localization signal requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins
- Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex
- In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate
- Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore
- Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex
- The protein encoded by this gene is a member of the importin beta family.