MilliporeSigma™ Mouse anti-Sortilin, Clone: 4E3.1,

Description

• Specifically detects Sortilin clone: 4E3.1 in Human, Mouse samples, and it is validated for Immunohistochemistry (Paraffin), Western Blotting Sortilin (UniProt Q99523; also known as 100kDa NT receptor, Glycoprotein 95, Gp95, Neurotensin receptor 3, NT3, NTR3) is encoded by the SORT1 (also known as LDLCQ6, NT3) gene (Gene ID 6272) in human
• Sortlin belongs to the a family of sorting receptors characterized by the Vps10p domain that creates a tunnel cavity with its ten-bladed β-propeller for the binding of soluble ligands
• The family members also contain a short cytoplasmic tail that harbors recognition motifs for subcellular sorting adaptor proteins
• Sortilin is reproted to bind PCSK9 in the trans-Golgi network with high-affinity in HepG2 cells
• Sort1 knockout mice exhibit reduced PCSK9 plasma concentration and altered PCSK9 subcellular localization in hepatocytes
• Sortilin is also found to colocalize with APP in neurites and facilitates APP processing toward the production of sAPPα, possibly by optimizing APP as a substrate for α-secretase cleavage
• Sortilin is initially produced with a signal peptide (a.a
• 1-33) and a propeptide (a.a
• 34-77) sequence, the removal of which yields the mature receptor (a.a
• 78-831) with a large luminal/extracellular (a.a
• 78-555) portion, a transmembrane region (a.a
• 756-778), and a short cytoplasmic tail (a.a.779-831)
• Similar to the other two family members (MPRs and SorLA), sortilin resides both on the membrane of intracellular vesicles and on the plasma membrane
• Altered sortilin expressions due to SORT1 genetic variations affect serum levels of low density lipoprotein cholesterol (LDL-C) and contribute to the LDL-C level quantitative trait locus 6 (LDLCQ6) and susceptibility to myocardial infarction.