MilliporeSigma™ HHV-8 LNA-1, Rat, Unlabeled, Clone: LN53,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABE1109MI-Each-of-1 | In Stock | ₹ 40,633.84 |
MABE1109MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 40,633.84
GST (18%): ₹ 7,314.091
Total Price: ₹ 47,947.931
Antigen
HHV-8 LNA-1
Classification
Monoclonal
Concentration
Please refer to lot specific datasheet.
Formulation
Purified rat monoclonal IgG2c in buffer containing 0.1M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.
Q9DUM3;Q9QR71
Immunogen
Affinity-purified GST fusions of HHV-8 LNA-1 C-terminal fragments (Kellam, P., et al. (1999). J. Virol. 73(6): 5149 5155).
Quantity
100 μg
Research Discipline
Epigenetics & Nuclear Function
Gene ID (Entrez)
AAD46501.1;YP_001129431
Target Species
Human
Form
Purified
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation
Clone
LN53
Conjugate
Unconjugated
Gene
ORF73
Host Species
Rat
Purification Method
Protein G Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone LN53 recognizes repetitive glutamic acid structural motifs EQEQE and EPEPE found in the Glu-rich repeat region of human Kaposi's sarcoma-associated herpesvirus (KSHV or HHV-8) and simian retroperitoneal fibromatosis herpesvirus (RFHV) Orf73 latency-associated nuclear antigen (LANA or LNA), respectively.
Content And Storage
Stable for 1 year at 2°-8°C from date of receipt.
Isotype
IgG2c κ
Related Products
Description
- Human herpesvirus-8//Kaposi's sarcoma-associated herpesvirus (HHV-8/KSHV) and macaque retroperitoneal fibromatosis herpesvirus (RFHV) were originally identified in in AIDS patients in association with Kaposi's sarcoma (KS) and in retroperitoneal fibromatosis (RF) tumor lesions of macaques with simian AIDS, respectively
- The most prominent protein expressed in cells latently infected with KSHV and RFHV is the orf73 gene product known as the latency-associated nuclear antigen (LANA or LNA)
- LANA is a nuclear protein that functions to ensure the maintenance of the viral genome by tethering the viral episomal DNA to host cell chromosomes
- LANA also regulates the cellular transcription program in host cells through interaction with a number of cellular proteins, including transcriptional regulators and known tumor suppressors, p53 and pRB
- In addtion, LANA directly influences the viral transcription program and helps maintain the latent state of the virus by inhibiting viral replication
- KSHV LANA consists of a serine- and proline-rich N-terminal domain with a nuclear localization signal (NLS), a chromatin-binding motif (CBM), and domains responsible for interaction with the transcription regulators, mSin3 complex and Sp1
- The central domain contains several highly repetitive acidic regions that vary in length and are responsible for the size variation of LANA proteins from different KSHV isolates that can range from 1003 to 1162 amino acids
- The proline-rich C-terminal third domain contains another NLS and is responsible for LANA dimerization and binding to the terminal repeats (TR) of the viral genomic DNA
- The C-terminal domain is responsible for interaction with tumor suppressors pRB and p53.