MilliporeSigma™ PRMT9, Mouse, Unlabeled, Clone: 128-29-1,
Manufacturer: MilliporeSigma™
CAS Number: 128-29-1
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABE1112MI-Each-of-1 | In Stock | ₹ 40,292.08 |
MABE1112MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 40,292.08
GST (18%): ₹ 7,252.574
Total Price: ₹ 47,544.654
Antigen
PRMT9
Classification
Monoclonal
Concentration
Please refer to lot specific datasheet.
Formulation
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.
Q6P2P2
Immunogen
GST-tagged recombinant protein corresponding to the N-terminal half of human PRMT9.
Quantity
100 μg
Research Discipline
Epigenetics & Nuclear Function
Gene ID (Entrez)
NP_612373
Target Species
Human
Form
Purified
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Clone
128-29-1
Conjugate
Unconjugated
Gene
PRMT9, PRMT10
Host Species
Mouse
Purification Method
Protein G Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone detected GST fusion containing recombinant human PRMT9 fragment a.a. 401-550, as well as endogenous human, but not mouse, PRMT9 in cell lysates (Yang, Y., et al. (2015). Nat. Commun. 6:6428). Epitope region is present in both spliced isoforms of human PRMT9 reported by UniProt (Q6P2P2).
Content And Storage
Stable for 1 year at 2°-8°C from date of receipt.
Isotype
IgG1 κ
Related Products
Description
- Putative protein arginine N-methyltransferase 9 (UniProt Q6P2P2; also known as Putative protein arginine N-methyltransferase 10) is encoded by the PRMT9 (also known as PRMT10) gene (Gene ID 90826) in human
- Mammalian protein arginine methyltransferases (PRMTs) catalyze the methylation of arginine residues on histone and non-histone proteins
- PRMTs are divided into three types based on their mode of activity
- Type I PRMTs (PRMT1–4, PRMT6, and PRMT8) transfer up to two methyl groups on the same terminal guanidino nitrogen of arginine to form ω-NG-monomethylarginine (MMA) and ω-NG,NG-asymmetric dimethylarginine (ADMA) residues
- Type II PRMTs (PRMT5 and PRMT9) transfer methyl groups on different terminal guanidino nitrogens to form μμα and ω-NG,N'G-symmetric dimethylarginine (SDMA) residues, while type III PRMT7 transfers only one single methyl group to form MMA residues
- PRMT9 is involved in alternative splicing regulation by modulating spliceosomal small nuclear ribonucleoprotein (snRNP) maturation in the cytoplasm
- PRMT9 complexes with the splicing factors SF3B2 (SAP145) and SF3B4 (SAP49) and mediates SF3B2 Arg508 modification via MMA and SDMA
- Similar to PRMT7, but unlike other PRMTs, PRMT9 contains a duplicated methyltransferase domain (a.a
- 137-446 and 530-845), with the N-terminal MTase acting as a funtional AdoMet-binding domain and the C-terminal MTase domain serving a pseudodimer function, mimicking the homodimer formation of other PRMTs needed for subtrate processing
- In addition, PRMT9 contains three tetratricopeptide repeats (TPR1-3; a.a
- 25-58, 67-100, 101-134) known to mediate protein-protein interactions.