MABE1177MI
MilliporeSigma™ PNKP, Mouse, Unlabeled, Clone: H101.2,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABE1177MI-Each-of-1 | In Stock | ₹ 38,003.00 |
MABE1177MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 38,003.00
GST (18%): ₹ 6,840.54
Total Price: ₹ 44,843.54
Antigen
PNKP
Classification
Monoclonal
Concentration
Please refer to lot specific datasheet.
Formulation
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Gene Accession No.
Q96T60
Immunogen
His-tagged recombinant full-length human PNKP.
Quantity
100 μg
Research Discipline
Epigenetics & Nuclear Function
Gene ID (Entrez)
NP_009185
Target Species
Bovine
Form
Purified
Applications
Immunoprecipitation, Western Blot
Clone
H101.2
Conjugate
Unconjugated
Gene
PNKP, AOA4, MCSZ
Host Species
Mouse
Purification Method
Protein G Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone H101.2 recognizes an epitope in the N-terminal region of PNKP.
Content And Storage
Stable for 1 year at 2°-8°C from date of receipt.
Isotype
IgG1 κ
Related Products
Description
- Bifunctional polynucleotide phosphatase/kinase (EC 2.7.1.78; EC 3.1.3.32; UniProt Q96T60; also known as DNA 5&prime-kinase/3&prime-phosphatase, Polynucleotide kinase-3&prime-phosphatase) is encoded by the PNKP (also known as AOA4, MCSZ) gene (Gene ID 11284) in human
- Polynucleotide kinase/phosphatase (PNKP) is a critical mammalian DNA repair enzyme that converts 3'-phosphate and 5'-hydroxyl at damaged DNA termini to generate 5&prime-phosphate and 3&prime-hydroxyl groups that are required for subsequent processing by DNA ligases and polymerases
- PNKP dephosphorylates the 3'-phosphate termini of single nucleotide gap DNAs produced by the endonuclease VIII-like DNA glycosylases NEILs prior to gap filling and ligation during base excision repair (BER)
- PNKP is also involved in the the nonhomologous end joining (NHEJ) pathway-mediated double-strand breaks repair by interacting with the NHEJ scaffolding protein XRCC4
- PNKP-knockdown in human cells leads to marked sensitization to spontaneous mutation and increased susceptibility to genotoxic agents
- Small molecules that selectively inhibit PNKP phosphatase activity sensitize human cells to DNA damage and mutations in the PNKP phosphatase domain are associated with the developmental neurological disorder MCSZ (Microcephaly, seizures, and developmental delay).