EphA2, Mouse anti-Human, Clone: F2-27, Millipore Sigma™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABS202625-Each-of-1 | In Stock | ₹ 17,118.26 |
MABS202625 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 17,118.26
GST (18%): ₹ 3,081.287
Total Price: ₹ 20,199.547
Antigen
EphA2
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
EPHA2;ECK
Immunogen
KP-2, KP-3, SUIT-2, and MIAPaCa-2 cells.
Quantity
25 μg
Research Discipline
Signaling
Gene ID (Entrez)
NP_004422
Target Species
Human
Form
Purified
Applications
Flow Cytometry, Immunoprecipitation, Inhibition Assays
Clone
F2-27
Dilution
Inhibition Analysis: A representative lot inhibited cancer cell invasion in a three-dimensional spheroid formation assay. (Tanaka, T., et. al. (2017). Transl Oncol. 10(4):476-484).Immunoprecipitation Analysis: A representative lot detected EphA2 in Immunoprecipitation applications (Tanaka, T., et. al. (2017). Transl Oncol. 10(4):476-484).Flow Cytometry Analysis: A representative lot detected EphA2 in Flow Cytometry applications (Tanaka, T., et. al. (2017). Transl Oncol. 10(4):476-484).
Gene Alias
Ephrin type-A receptor 2;EC: 2.7.10.1;Epithelial cell kinase;Tyrosine-protein kinase receptor ECK
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone F2-27 specifically detects Ephrin type -A receptor 2 in human.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG1 κ
Related Products
Description
- Anti-EphA2, clone F2-27, Cat
- No
- MABS2026, is a mouse monoclonal antibody that detects Ephrin type-A receptor 2 and has been tested for use in Flow Cytometry, Immunoprecipitation, and Inhibition Assay
- Ephrin type-A receptor 2 (UniProt: P29317; also known as EC: 2.7.10.1, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK) is encoded by the EPHA2 (also known as ECK) gene (Gene ID: 1969) in human
- Ephrin type-A receptor 2 is a homodimeric single-pass type I membrane protein that is expressed in brain and glioma tissue and in glioma cell lines
- It is also highly expressed in tissues that contain a high proportion of epithelial cells
- Its levels are shown to be up-regulated by UV irradiation via a TP53-independent, MAPK-dependent mechanism
- Ephrin type-A receptor 2 serves as a receptor tyrosine kinase that binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells
- It is involved in the regulation of migration, integrin-mediated adhesion, and in proliferation and differentiation of cells
- Ephrin type-A receptor 2 is phosphorylated on tyrosine residues upon binding and activation by EFNA1
- Phosphorylation on Tyr 588 and Tyr 594 is reported to be essential for binding VAV2 and VAV3 while phosphorylation on Tyr 735 and Tyr 930 are required for binding PI3-kinase p85 subunit
- It is synthesized with a signal peptide (aa 1-23) that is cleaved off in the mature form
- The mature form has an extracellular domain (aa 24-537), a short transmembrane domain (aa 538-558), and a cytoplasmic domain (aa 559-976)
- Two isoforms of Ephrin type-A receptor 2 have been described that are produced by alternative splicing
- EphA2 is reported to be overexpressed in skin tumors murine models and its overexpression may represent a compensatory feedback mechanism during tumorigenesis
- (Ref.: Guo, H., et al
- (2006)
- Cancer Res
- 66(14); 7050-7058).